Angiomotin ELISA Kit
Quick Overview for Angiomotin ELISA Kit (ABIN2042849)
Target
See all Angiomotin (AMOT) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 0.31 ng/mL
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Brand
- BioAssay™
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Sensitivity
- < 0.112 ng/mL
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Angiomotin (AMOT) ELISA Kit
AMOT Reactivity: Mouse Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Angiomotin (AMOT) ELISA KitAMOT Reactivity: Human Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Angiomotin (AMOT) ELISA KitAMOT Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- Angiomotin (AMOT) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiomotin (AMOT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Angiomotin (AMOT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Angiomotin (AMOT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Angiomotin (AMOT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- Angiomotin (AMOT)
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Pathways
- Cell-Cell Junction Organization, Regulation of Cell Size
Target See all Angiomotin (AMOT) ELISA Kits
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